This study presents the optimization of a simple HPLC-UV method for the determination of asenapine maleate in rat plasma. Ion pair separation followed by UV detection was performed on deproteinized rat plasma samples. The separation was carried out on a cosmosil C-18 column (250 mm × 4.6 mm, 5 µm) with UV detection at 232 nm. The mobile phase contained acetonitrile: potassium dihydrogen phosphate buffer pH 3.2 (60:40 v/v). The mobile phase was run isocratically. The flow rate of the mobile phase was maintained at 1 ml/min. The linearity of the calibration curve was obtained in the concentration range of 10 to 100 µg/ml for asenapine maleate and coefficient of correlation (R2) was found to be 0.997. The lowest limit of detection and quantification was 0.188 and 0.57 ng/ml respectively. No endogenous substances were found to interfere with the peaks of drug and plasma. The intra-day and inter-day coefficient of variations was less for all the selected concentrations. This method was time efficient and samples are easy to prepare with minimum dilution. So, it can be applied for monitoring asenapine maleate in rat plasma.
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